Content
Chapter 1 Introduction to Basic Safety in Bio-Laboratory 4
1.1 Introduction to biosafety levels and general precautions in bioscience laboratory 4
1.2 Safety guidelines on water usage in bioscience laboratory 6
1.3 Safety guidelines on electricity usage in bioscience laboratory 8
1.4 Safety guidelines on gas usage in bioscience laboratory 10
1.5 Safety guidelines on sound usage in bioscience laboratory 12
1.6 Safety guidelines on light usage in bioscience laboratory 13
Chapter 2 Standard Operations and Safety Precautions of General Apparatus in Bio-Laboratory 16
2.1 Standard operations and safety precautions of autoclave usage.......................................................................................................16
2.2 Standard operations and safety precautions of oven usage 20
2.3 Standard operations and safety precautions of ultra-low temperature freezer 22
2.4 Standard operations and safety precautions of liquid nitrogen container 23
2.5 Standard operations and safety precautions of centrifuge .......................................................................................................24
2.6 Standard operations and safety precautions of biosafety cabinet 28
2.7 Standard operations and safety precautions of clean bench 30
2.8 Standard operations and safety precautions of fume hood ........................................................................................................32
2.9 Standard operations and safety precautions of UV analyzer 33
2.10 Standard operations and safety precautions of laser 35
2.11 Standard operations and safety precautions of laser power meter 37
2.12 Standard operation and safety precaution of biological signal acquisition and analysis system 39
Chapter 3 General Safety Guidelines for Common Biochemical Reagents Usages 42
3.1 Toxic reagents 42
3.2 Chemical unstable reagents 49
3.3 Animal anesthetics 53
Chapter 4 Storage and Handling Guidelines of Experimental Biomaterials 55
4.1 Storage and handling guideline of biomaterials in the life science laboratory 55
4.2 Blood samples of human and animal 61
4.3 Microorganism 63
4.4 Biomacromolecule 64
Chapter 5 Bio-science Safety, Emergency Care and First Aid 66
5.1 Bio-laboratory potential fire accidents 66
5.2 Explosive Accidents 69
5.3 Poisoning and burn accidents 71
5.4 First aid medical box in bio-laboratory 75
References 76
內容試閱：
This procedure guideline was developed by Experimental Teaching Center for Life Science and Technology of Huazhong University of Science and Technology, College of Life Science and Technology to provide sufficient information on safety protection of workers and the surrounding environment in bioscience laboratory. This guideline is expected to be revised up to date guaranteeing that regulations, policies and procedures follow applicable and safe standards.All users in bioscience laboratories of educational institutions, including researchers, teachers, and students, are required to study and implement safety precautions to avoid safety accidents. This book aims to protect operators, experiment equipment and the research environment to reduce or eliminate the potential of biohazards. Neither any harms to the health of the operators nor any intentional failure of equipment is expected during bioscience experiments. Also, the research environment shall be kept fine for continuous work. Especially targeted to be applied to laboratories in general bio-laboratories for undergraduate and postgraduate education, this manual illustrates detailed procedure and guidelines for laboratory water, electricity, gas, sound, light usage and for some common apparatus. The contents are formulated on fundamental laboratory safety knowledge, standard equipment operations, the main properties of chemicals, and past laboratory experiences. The contents are concise and practical. In addition to text description, there are also standard operation procedure figures in standard operations of equipment part. Safety precautions are illustrated with figures. Therefore, this guideline is excellent with pictures and texts and are easy to understand. In addition, this guideline is scripted mainly based on Laboratory Biosafety Manual 3rd Edition, WHO, General requirements for laboratory biosafetyGB19489-2008, the Control of Substances Hazard to Health COSHH http：www.hse.gov.uk, the Globally Harmonized System of Classification and Labeling of Chemicals GHS etc. Moreover, this book is edited by first-line researchers in bioscience laboratories who have rich research and teaching experience, so the contents is specific and has practice guiding significance for users of bioscience laboratories.
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