赵春贞：副教授、硕士研究生导师。山乐约理学会教学与科普专业委员会副主任委员、中国药理学会教学与科普专业委员会委员、山东药理学会理事、山东毒理学会理事。山东省研究生优质课程负责人、中国医药研究生在线课程群建设项目负责人。主持、参与国家自然科学基金项目4项，发表SCI收录论文20余篇。主编、参编教材7部。授权国家发明专利3项。李成檀：副教授、硕士研究生导师，浙江省高校优秀青年教师资助计划人选。从事药理学教学16年，主讲药理学、药理学实验等课程。参编《药理学》《临床药理学》等教材、专著5部，获浙江省教学成果二等奖，主持、参与各级教学改革项目、科研项目10余项，在Ageing Res Rev、Neurobiol Aging等期刊发表论文20余篇。王雅娟：副教授、硕士研究生导师。任中国药理学会抗炎免疫药理学分会青年委员、安徽省药理学会抗炎免疫药理学专业委员会第一届委员会委员。从事教学工作近二十年，承担《药理学》临床药理学》《实验动物学》等课程的教学及相关科研工作。主持并参与了多项国家级、省级教研和科研项目，参与安徽省高等学校一流教材《护理药理学》的编写工作。
Chapter 1 General pharmacology 187
Section 1 Basic knowledge of experimental animals 187
Section 2 Common techniques for experimental animals 192
Section 3 Ethics of experimental animals 202
Section 4 Determination of plasma half life of sulfadiazine 204
Section 5 Determination of LD50 of procaine 210
Section 6 The influence of liver function on drug action 215
Section 7 Effects of different dosage on drug action 220
Section 8 Effects of different administration routes on the action of
magnesium sulfate 224
Section 9 Determination of IC50 of drugs in vitro 228
Chapter 2 Pharmacology of efferent nervous system 234
Section 1 Dose-effect relationship of acetylcholine 234
Section 2 The effect of efferent nervous system drugs on isolated
intestinal smooth muscle 238
Section 3 The effect of efferent nervous system drugs on the pupil of rabbits 243
Section 4 Inhibition of atropine on smooth muscle in mice 247
Section 5 Poisoning and rescue of organophosphorus pesticides 253
Chapter 3 Central nervous system pharmacology 258
Section 1 Analgesia experiment of drugs 258
Section 2 The effect of chlorpromazine on electric stimulation-induced
agitation in mice 263
Section 3 Effects of aspirin and chlorpromazine on body temperature 266
Section 4 Comparison of the effects of pancuronium bromide and
succinylcholine on muscle relaxation in rabbits 272
Section 5 Anticonvulsant effect of phenobarbital sodium in mice 276
Section 6 The effect of drugs on resisting nikethamide-induced convulsions 282
Section 7 Anxiolytic effects of diazepam 287
Section 8 Rescue effect of nikethamide on morphine induced respiratory
depression 292
Section 9 Local and systemic effects of procaine 296
Chapter 4 Cardiovascular system pharmacology 301
Section 1 Effect of efferent nervous system drugs on blood pressure in rabbits 301
Section 2 The effect of lidocaine on arrhythmia induced by electrical
stimulation 307
Section 3 The therapeutic effect of antiarrhythmic drugs on BaCl2-induced
arrhythmia 310
Section 4 The effect of cardiac glycosides on the relaxation and contraction of
detached frog hearts 314
Section 5 Effects of propranolol on hypoxia tolerance in mice 319
Chapter 5 Hormonal visceral pharmacology 324
Section 1 Antagonistic effect of protamine sulfate on heparin anticoagulant
activity 324
Section 2 Reaction and rescue of insulin overdose 328
Section 3 The antagonistic effect of aspirin on thrombosis formation 332
Section 4 The effect of oxytocin on uterine smooth muscle in vitro in
guinea pigs 337
Section 5 The effect of diuretics on animal urine output 341
Section 6 The effect of hydrocortisone on ear swelling induced by paraxylene
in mice 345
Section 7 Effects of magnesium sulfate and raw rhubarb on intestinal
propulsion in mice 350
Section 8 The effect of drugs on coagulation time in mice 354
Section 9 Observation of antitussive effect of pentoverine 359
Chapter 6 Pharmacology of chemotherapy drugs 364
Section 1 Toxic reactions of streptomycin and its relief 364
Section 2 Determination of blood concentration of cefradine 367
Section 3 Hemolysis test of injection 371
Section 4 Pyrogen examination of injection 376
Section 5 The inhibitory effect of doxorubicin on tumor cell growth in vitro 381
Section 6 The in vitro antibacterial effect of penicillin G 385
Chapter 7 Experimental design 392
Section 1 The objective and significance of experimental design 392
Section 2 The basic elements and principles of experimental design 393
Section 3 The main content and method of experimental design 398
Section 4 Types of experimental design 400
Section 5 The procedure and requirement of experimental design 402
Chapter 8 Case analysis 405
Case 1 405
Case 2 406
Case 3 407
Case 4 407
Chapter 9 Common experimental instruments 409
Section 1 Information integrated signal acquisition and processing system 409
Section 2 Spectrophotometer 414
Section 3 Physiological and pharmacological electronic stimulators 417
Appendix 422
Appendix 1 Conversion of drug concentration and dosage in pharmacological experiments 422
Appendix 2 Composition and preparation of common physiological solutions 425
Appendix 3 Drug dosage form and prescription 426
Appendix 4 Experimental report writing 430
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