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梨是全球性的重要水果之一。但梨果实软化、品质下降影响了其采后贮藏性和商品性,制约了其在市场上的流通和供应期。果实质地是影响果实品质和消费者选择的一项重要因素。果实软化和细胞壁降解密切相关,果实硬化主要和木质素积累有关。在本书中,我们主要集中介绍了细胞壁降解(第2章,第8至第9章),木质素合成(第1章,第3至第6章),以及采前套袋(第7章)对梨果实质地变化特性的影响。讲述了质地相关基因的克隆及转基因功能验证。
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本书可以拓展人们以往对果实品质的观念,了解到果实不仅具有软化质地,而且也会发生木质素,并且了解木质化发生的机制。本选题针对我国梨、桃、枇杷等果实出现的采后木质化现象,严重影响了果实的外观品质、食用品质和贮藏品质等问题提出的。拟深入分析影响果实木质化发生的功能基因及转录因子等协同调控的分子机制,完善了果实质地变化的理论依据,为生产上解决果实采后贮藏提供科学依据。
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| 關於作者: |
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杨绍兰,1998年-2002年就读于西北农林科技大学,获得学士学位,2002年-2007年就读于浙江大学,获得博士学位。2007年至今在青岛农业大学园艺学院工作至今。目前主要从事果实品质特性及调控机理研究。主持参与多项国家和省部级项目。已在《Postharvest and BiologyTechnology》、《Journal of Plant Growth and Regulation》、《Journal ofAgricultural and food chemistry》、《Plant Growth and Regulation》《Genes》和《园艺学报》等国内外期刊发表论文60余篇。
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| 目錄:
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Contents
Chapter 1Ligninassociated Genes Expression Pattern in
Pear Fruit
I Introduction
II Materials and Methods
III Results
IV Discussion
Chapter 2The Expression Pattern of Seven PcEXPA
Genes of Chili and Whangkeumbae
Pear during Fruit Postharvest Storage
I Introduction
II Materials and Methods
III Results
IV Discussion
Chapter 3Effects of Preharvest Calcium Treatment on the
Storage Characters of Hard End Whangkeumbae
Pear and the Expression Analysis of PpEXPA2
I Introduction
II Materials and Methods
III Results and Analysis
IV Discussion
Chapter 4Altering PpCAD2 and PpEXP2 Gene Expression
Impacts Lignification in Transgenic
Tobacco Plants
I Introduction
II Materials and Methods
III Results
IV Discussion
V Conclusions
Chapter 5The Heterologous Expression of PpCAD2 in
Tomato MicroTom Verified Its Function in
Affecting Lignin Content
I Introduction
II Materials and Methods
III Results and Discussion
IV Conclusions
Chapter 6PpNAC187 Was Involved in the Lignin
Synthesis in Whangkeumbae Pear
Pyrus pyrifoliaFruit
I Introduction
II Materials and Methods
III Results
IV Discussion
Chapter 7Effect of Preharvest Bagging on Fruit Storage
Characteristics of Chili Pear Fruit
I Introduction
II Materials and Methods
III Results and Discussion
IV Conclusions
Chapter 8The Gene Cloning and Analysis of Expression Pattern
of Endo1,4glucanase Gene during Chili Pear
Fruit Development and Maturation
I Introduction
II Results and Analysis
III Discussion
IV Conclusions
Chapter 9Expression of Two Endo1,4glucanase Genes
during Fruit Ripening and Softening of
Two Pear Varieties
I Introduction
II Materials and Methods
III Results
IV Discussion
References
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Fruit texture is a very important factor that affects the fruit quality,and to a certain extent,it is an indicator for consumers to choose fruits Fruit softening is mainly related with the cell wall degradation,and the fruit hardening is often concerning to the lignin accumulation In this book,we mainly focused on the effect of cell wall degradationChapter 2,Chapters 8-9,lignin synthesisChapter 1,Chapters 3-6and preharvest baggingChapter 7on the pearPyrus pyrifoliafruit texture change characters The related genes were cloned and the gene function were carried out by transgenic methods.
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